Mouse IgG1/IgG2a/IgG3 Three-Color FluoroSpot

This optimized ELISPOT assay reliably detects mouse IgG1, IgG2a, and IgG3 secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.

This assay utilizes the Capture and Detection Kits listed below.

 

Available in 2 and 5 plate sets.

 

Components

Catalog #

 

Ig Capture Kit

mB3F

 

IgG1 Detection Kit

mB25

 

IgG2a Detection Kit

mB33

 

IgG3 Detection Kit

mB50

 
Scanning and analysis requires an analyzer with fluorescent capabilities. CTL recommends any of the following Series 5 or Series 6 readers running ImmunoSpot Software version 5.3 or higher: S5UV, FluoroCore, Ultra, Universal, Ultimate.



Click to view details
Mouse IgG1/IgG2a/IgG3 Three-Color FluoroSpotMouse IgG1/IgG2a/IgG3 Three-Color FluoroSpot

Details

ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!

Capture Kit
Includes Capture Antibodies, serum- free medium, low autofluorescence PVDF plates, polyclonal B cell activator (B-Poly-S™), and all diluents.

Detection Kit
Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted antibodies of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific Ig class/IgG subclass by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.

Datasheets

SDS

CTL Europe GmbH 
Hans-Boeckler-Str. 19-29 | 53225 Bonn, Germany
+49 228 85 44 78-0 | +49 228 85 44 78-22 Fax                     
Privacy Policy | Imprint | Sitemap
ctl-europe@immunospot.eu | www.immunospot.eu

© 2017 CTL Europe GmbH