Working with Peptides and Peptide Pools

  • Ott, P. A., B. A. Herzog, S. Quast, H. H. Hofstetter, B. O. Boehm, M. Tary-Lehmann, I. Durinovic-Bello, B. R. Berner, and P. V. Lehmann. 2005. Islet-cell antigen-reactive T cells show different expansion rates and Th1/Th2 differentiation in type 1 diabetic patients and healthy controls. Clin. Immunol. 115:102.
  • Herzog, B. A., P. A. Ott, M. T. Dittrich, S. Quast, A. Y. Karulin, H. Kalbacher, W. Karges, M. Tary-Lehmann, P. V. Lehmann, B. O. Boehm, and I. Durinovic-Bello. 2004. Increased in vivo frequency of IA-2 peptide-reactive IFNgamma+/IL-4- T cells in type 1 diabetic subjects. J. Autoimmun. 23:45. <abstract>
  • Kleen, T. O., R. Asaad, S. J. Landry, B. O. Boehm, and M. Tary-Lehmann. 2004. Tc1 effector diversity shows dissociated expression of granzyme B and interferon-gamma in HIV infection. AIDS 18:383.
  • Ott, P. A., M. T. Dittrich, B. A. Herzog, R. Guerkov, P. A. Gottlieb, A. L. Putnam, I. Durinovic-Bello, B. O. Boehm, M. Tary-Lehmann, and P. V. Lehmann. 2004. T cells recognize multiple GAD65 and proinsulin epitopes in human type 1 diabetes, suggesting determinant spreading. J. Clin. Immunol. 24:327.
  • Ott, P. A., B. R. Berner, B. A. Herzog, R. Guerkov, N. L. Yonkers, I. Durinovic-Bello, M. Tary-Lehmann, P. V. Lehmann, and D. D. Anthony. 2004. CD28 costimulation enhances the sensitivity of the ELISPOT assay for detection of antigen-specific memory effector CD4 and CD8 cell populations in human diseases. J. Immunol. Methods 285:223.
  • Anthony, D. D., and P. V. Lehmann. 2003. T-cell epitope mapping using the ELISPOT approach. Methods 29:260. Kreher, C. R., M. T. Dittrich, R. Guerkov, B. O. Boehm, and M. Tary-Lehmann. 2003. CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays. J. Immunol. Methods 278:79.
  • Kreher, C. R., M. T. Dittrich, R. Guerkov, B. O. Boehm, and M. Tary-Lehmann. 2003. CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays. J. Immunol. Methods 278:79.
  • Stern, B. V., B. O. Boehm, and M. Tary-Lehmann. 2002. Vaccination with tumor peptide in CpG adjuvant protects via IFN-gamma-dependent CD4 cell immunity. J. Immunol. 168:6099.
  • Hesse, M. D., A. Y. Karulin, B. O. Boehm, P. V. Lehmann, and M. Tary-Lehmann. 2001. A T cell clone's avidity is a function of its activation state. J. Immunol. 167:1353.
  • Heeger, P. S., A. Valujskikh, and P. V. Lehmann. 2000. Comprehensive assessment of determinant specificity, frequency, and cytokine signature of the primed CD8 cell repertoire induced by a minor transplantation antigen. J. Immunol. 165:1278.
  • Pelfrey, C. M., R. A. Rudick, A. C. Cotleur, J. C. Lee, M. Tary-Lehmann, and P. V. Lehmann. 2000. Quantification of self-recognition in multiple sclerosis by single-cell analysis of cytokine production. J. Immunol. 165:1641.
  • Targoni, O. S., and P. V. Lehmann. 1998. Endogenous myelin basic protein inactivates the high avidity T cell repertoire. J. Exp. Med. 187:2055.
  • Chu, R. S., O. S. Targoni, A. M. Krieg, P. V. Lehmann, and C. V. Harding. 1997. CpG oligodeoxynucleotides act as adjuvants that switch on T helper 1 (Th1) immunity. J. Exp. Med. 186:1623. Forsthuber, T., H. C. Yip, and P. V. Lehmann. 1996. Induction of TH1 and TH2 immunity in neonatal mice. Science 271:1728.
  • Forsthuber, T., H. C. Yip, and P. V. Lehmann. 1996. Induction of TH1 and TH2 immunity in neonatal mice. Science 271:1728.
  • Lehmann, P. V., F. Cardinaux, E. Appella, S. Muller, F. Falcioni, L. Adorini, and Z. A. Nagy. 1989. Inhibition of T cell response with peptides is influenced by both peptide-binding specificity of major histocompatibility complex molecules and susceptibility of T cells to blocking. Eur. J. Immunol. 19:1071.
  • Nagy, Z. A., P. V. Lehmann, F. Falcioni, S. Muller, and L. Adorini. 1989. Why peptides? Their possible role in the evolution of MHC-restricted T-cell recognition. Immunol. Today 10:132.
  • Adorini, L., S. Muller, F. Cardinaux, P. V. Lehmann, F. Falcioni, and Z. A. Nagy. 1988. In vivo competition between self peptides and foreign antigens in T-cell activation. Nature 334:623.
  • Adorini, L., A. Sette, S. Buus, H. M. Grey, M. Darsley, P. V. Lehmann, G. Doria, Z. A. Nagy, and E. Appella. 1988. Interaction of an immunodominant epitope with Ia molecules in T-cell activation. Proc. Natl. Acad. Sci. U. S. A 85:5181.
  • Pelfrey, C. M., Moldovan, I. R., Cotleur, A. C., Zamor, N, Rudick, R. A., Effects of sex hormones on costimulatory molecule expression in multiple sclerosis. J Neuroimmunol. 2005 Oct;167(1-2):190-203. <abstract>
  • Moldovan, I.R., Rudick, R.A., Cotleur, A.C., Born S.E., Lee, J.C., Karafa, M.T., Pelfrey, C.M. Longitudinal single-cell cytokine responses reveal recurrent autoimmune myelin reactivity in relapsing-remitting multiple sclerosis patients. Mult Scler. 2005 Jun;11(3):251-60. <abstract>
  • Pelfrey C. M., Moldovan I. R., Epitope mapping in multiple sclerosis using the ELISPOT assay. Methods Mol Biol. 2005;302:219-36. <abstract>
  • Moldovan, I.R., Rudick, R.A., Cotleur, A.C., Born S.E., Lee, J.C., Karafa, M.T., Pelfrey, C.M. Interferon gamma responses to myelin peptides in multiple sclerosis correlate with a new clinical measure of disease progression. J Neuroimmunol. 2003 Aug;141(1-2):132-40. <abstract>