਀    㰀栀攀愀搀㸀 CEF Peptide Pools for activating CD8 memory cells਀        㰀䰀䤀一䬀 琀礀瀀攀㴀∀琀攀砀琀⼀挀猀猀∀ 爀攀氀㴀∀猀琀礀氀攀猀栀攀攀琀∀ 栀爀攀昀㴀∀⸀⸀⼀猀琀礀氀攀⸀挀猀猀∀㸀 ਀            㰀洀攀琀愀 挀漀渀琀攀渀琀㴀∀䌀䔀䘀 瀀攀瀀琀椀搀攀 瀀漀漀氀猀Ⰰ 攀氀椀猀瀀漀琀∀ 渀愀洀攀㴀∀欀攀礀眀漀爀搀猀∀㸀㰀⼀栀攀愀搀㸀 ਀        㰀琀愀戀氀攀 椀搀㴀∀吀愀戀氀攀㄀∀ 挀攀氀氀匀瀀愀挀椀渀最㴀∀　∀ 挀攀氀氀倀愀搀搀椀渀最㴀∀　∀ 眀椀搀琀栀㴀∀㄀　㄀　∀ 愀氀椀最渀㴀∀氀攀昀琀∀ 戀漀爀搀攀爀㴀∀　∀㸀 ਀                㰀琀搀 眀椀搀琀栀㴀∀㔀　∀㸀㰀⼀琀搀㸀 ਀                㰀琀搀 眀椀搀琀栀㴀∀㌀　　∀㸀㰀⼀琀搀㸀 ਀                㰀琀搀 眀椀搀琀栀㴀∀㌀　　∀㸀㰀⼀琀搀㸀 ਀                㰀琀搀 眀椀搀琀栀㴀∀㈀㜀㠀∀㸀㰀⼀琀搀㸀 ਀                㰀琀搀 眀椀搀琀栀㴀∀㐀　∀㸀㰀⼀琀搀㸀 ਀            㰀琀爀㸀 ਀                㰀琀搀 瘀䄀氀椀最渀㴀∀戀漀琀琀漀洀∀ 眀椀搀琀栀㴀∀㄀　　　∀ 挀漀氀匀瀀愀渀㴀∀㠀∀㸀㰀䤀䴀䜀 琀椀琀氀攀㴀∀䔀䰀䤀匀倀伀吀Ⰰ 瀀攀瀀琀椀搀攀猀∀ 猀爀挀㴀∀⸀⸀⼀椀洀愀最攀猀⼀栀攀愀搀攀爀ⴀ椀洀洀甀渀漀猀瀀漀琀ⴀ昀氀礀攀爀⸀最椀昀∀㸀㰀⼀琀搀㸀 ਀            㰀琀爀㸀 ਀                㰀琀搀 眀椀搀琀栀㴀∀㈀　∀ 栀攀椀最栀琀㴀∀㈀　∀㸀㰀⼀琀搀㸀 ਀                        䌀䔀䘀 倀攀瀀琀椀搀攀 倀漀漀氀猀☀渀搀愀猀栀㬀吀栀攀 倀漀猀椀琀椀瘀攀 䌀漀渀琀爀漀氀 昀漀爀 䄀挀琀椀瘀愀琀椀渀最 䌀䐀㠀 䴀攀洀漀爀礀 䌀攀氀氀猀㰀⼀䄀㸀㰀⼀琀搀㸀 ਀                㰀琀搀 眀椀搀琀栀㴀∀㐀　∀㸀㰀⼀琀搀㸀 ਀            㰀琀爀㸀 ਀                㰀琀搀 瘀䄀氀椀最渀㴀∀戀漀琀琀漀洀∀ 眀椀搀琀栀㴀∀㤀㘀　∀ 挀漀氀匀瀀愀渀㴀∀㜀∀ 栀攀椀最栀琀㴀∀㄀　∀㸀㰀䤀䴀䜀 猀爀挀㴀∀⸀⸀⼀椀洀愀最攀猀⼀昀氀礀攀爀ⴀ琀漀瀀ⴀ氀椀渀攀⸀最椀昀∀㸀㰀⼀琀搀㸀 ਀            㰀⼀琀爀㸀 ਀                㰀琀搀 眀椀搀琀栀㴀∀㔀　∀ 爀漀眀匀瀀愀渀㴀∀㈀∀㸀㰀⼀琀搀㸀 ਀                㰀琀搀 瘀䄀氀椀最渀㴀∀琀漀瀀∀ 愀氀椀最渀㴀∀氀攀昀琀∀ 眀椀搀琀栀㴀∀㌀　　∀ 挀漀氀匀瀀愀渀㴀∀㄀∀ 爀漀眀匀瀀愀渀㴀∀㈀∀㸀

CTL, in collaboration with PANATecs, offers two peptide pools that cover HLA class I restricted T cell epitopes of Cytomegalovirus, Epstein-Barr Virus, and Flu Virus (CEF peptides).਀                    㰀瀀㸀 Most humans have been infected and have developed CD8 positive cell responses ਀                    琀漀 琀栀攀猀攀 瘀椀爀甀猀攀猀㬀 椀渀 愀搀搀椀琀椀漀渀Ⰰ 琀栀攀 搀攀琀攀爀洀椀渀愀渀琀猀 栀愀瘀攀 戀攀攀渀 猀攀氀攀挀琀攀搀 琀漀 挀漀瘀攀爀  common HLA-alleles. For these reasons, these peptides will stimulate the CD8 ਀                    洀攀洀漀爀礀 挀攀氀氀猀 漀昀 洀漀猀琀 栀甀洀愀渀 猀甀戀樀攀挀琀猀⸀ 䌀䔀䘀 瀀攀瀀琀椀搀攀猀 愀爀攀 琀栀攀爀攀昀漀爀攀 愀渀 椀搀攀愀氀  positive control when testing PBMC in ELISPOT assays, intracellular cytokine ਀                    猀琀愀椀渀椀渀最Ⰰ 挀礀琀漀欀椀渀攀 戀攀愀搀 愀爀爀愀礀猀Ⰰ 䔀䰀䤀匀䄀 漀爀 愀渀礀 漀琀栀攀爀 琀攀猀琀 猀礀猀琀攀洀 琀栀愀琀 愀椀洀猀 昀漀爀  detecting antigen-specific T cell recall responses.਀                    㰀瀀㸀吀爀愀搀椀琀椀漀渀愀氀氀礀 瀀栀礀琀漀栀攀洀愀最最氀甀琀椀渀椀渀 ⠀倀䠀䄀⤀Ⰰ 倀䴀䄀ⴀ椀漀渀漀洀礀挀椀渀Ⰰ 漀爀 愀渀琀椀ⴀ䌀䐀㌀ 愀渀琀椀戀漀搀礀 栀愀瘀攀  been used as a positive control for ELISPOT assays. However, such stimuli are ਀                        瀀漀氀礀挀氀漀渀愀氀 愀渀搀 栀攀渀挀攀 琀漀漀 猀琀爀漀渀最 琀漀 爀攀昀氀攀挀琀 漀渀 愀渀琀椀最攀渀ⴀ猀瀀攀挀椀昀椀挀 吀 挀攀氀氀  responses. PHA and PMAionomycin activate many cell types in addition to T ਀                        挀攀氀氀猀⸀ 吀栀攀 㰀椀㸀瀀攀爀 挀攀氀氀㰀⼀椀㸀 cytokine production induced by the former is significantly more than ਀                    愀渀琀椀最攀渀ⴀ琀爀椀最最攀爀攀搀 挀礀琀漀欀椀渀攀 瀀爀漀搀甀挀琀椀漀渀 戀礀 吀 挀攀氀氀猀⸀ 吀栀攀 䌀䔀䘀 瀀攀瀀琀椀搀攀 瀀漀漀氀猀  circumvent these problems.਀                    㰀瀀㸀䌀䔀䘀 瀀攀瀀琀椀搀攀猀 猀琀椀洀甀氀愀琀攀 漀渀氀礀 䌀䐀㠀⬀ 挀攀氀氀猀⸀ 䤀渀 愀搀搀椀琀椀漀渀 琀漀 甀猀椀渀最 䌀䔀䘀 瀀攀瀀琀椀搀攀猀 愀猀 愀  positive control for CD8 cell functionality, they can also be used to control ਀                        昀漀爀 琀栀攀 愀搀攀焀甀愀挀礀 漀昀 䌀䐀㠀 吀 挀攀氀氀 搀攀瀀氀攀琀椀漀渀⸀

23 CEF Class I Peptide Pool

਀                    㰀瀀㸀吀栀攀 挀氀愀猀猀椀挀 ㈀㌀ 䠀䰀䄀 挀氀愀猀猀 䤀ⴀ爀攀猀琀爀椀挀琀攀搀 䌀䔀䘀 吀 挀攀氀氀 攀瀀椀琀漀瀀攀猀⸀ 吀栀攀 瀀漀漀氀 挀漀瘀攀爀猀  determinants restricted by 11 HLA alleles: A1, A2, A3, A11, A24, A68, B7, B8, ਀                        䈀㈀㜀Ⰰ 䈀㌀㔀Ⰰ 愀渀搀 䈀㐀㐀⸀ 䴀漀猀琀 䌀愀甀挀愀猀椀愀渀 椀渀搀椀瘀椀搀甀愀氀 攀砀瀀爀攀猀猀 愀琀 氀攀愀猀琀 漀渀攀 漀昀 琀栀攀猀攀  alleles and the 23 peptides selected cover CD8 cell epitopes in most ਀                        䌀愀甀挀愀猀椀愀渀猀⸀ 嬀㄀崀㰀⼀瀀㸀 ਀                㰀琀搀 爀漀眀匀瀀愀渀㴀∀㈀∀㸀㰀⼀琀搀㸀 ਀            㰀⼀琀爀㸀 ਀                㰀琀搀 瘀䄀氀椀最渀㴀∀琀漀瀀∀ 眀椀搀琀栀㴀∀㌀　　∀㸀

32 CEF Class I Peptide Pool

਀                    㰀倀㸀 The extended peptide pool consisting of 32 HLA class I restricted CEF T cell ਀                    攀瀀椀琀漀瀀攀猀Ⰰ 愀渀搀 挀漀瘀攀爀椀渀最 ㄀㔀 䠀䰀䄀 挀氀愀猀猀 䤀 愀氀氀攀氀攀猀Ⰰ 椀渀 瀀愀爀琀椀挀甀氀愀爀 䠀䰀䄀㨀 䄀㄀Ⰰ 䄀㈀Ⰰ  A0201, A3, A11, A24, A68, A6081, B7, B8, B18, B27, B35,B44, B0702. Because the ਀                    挀氀愀猀猀椀挀 ㈀㌀ 䌀䔀䘀 瀀攀瀀琀椀搀攀猀 昀愀椀氀攀搀 琀漀 攀氀椀挀椀琀 䌀䐀㠀 挀攀氀氀 爀攀挀愀氀氀 爀攀猀瀀漀渀猀攀猀 椀渀 愀氀氀  Caucasians, the original peptide pool by Currier, et al. was extended by nine ਀                    瀀攀瀀琀椀搀攀猀 琀栀愀琀 挀漀瘀攀爀 搀攀琀攀爀洀椀渀愀渀琀猀 愀渀搀 洀漀爀攀 䠀䰀䄀 愀氀氀攀氀攀猀⸀ 吀栀攀 攀砀琀攀渀搀攀搀 ㌀㈀ 瀀攀瀀琀椀搀攀  pool elicits recall responses in most humans.਀                    㰀倀㸀䰀䤀吀㨀 嬀㄀崀 㰀䔀䴀㸀䄀 瀀愀渀攀氀 漀昀 䴀䠀䌀 挀氀愀猀猀 䤀 爀攀猀琀爀椀挀琀攀搀 瘀椀爀愀氀 瀀攀瀀琀椀搀攀猀 昀漀爀 甀猀攀 愀猀 愀 焀甀愀氀椀琀礀  control for vaccine trial ELISPOT assays: J.R. Currier, et al.; J.Immunol. ਀                            䴀攀琀栀漀搀猀 ㈀㘀　Ⰰ ㄀㔀㜀 ⠀㈀　　㈀⤀㰀⼀䔀䴀㸀㰀⼀倀㸀 ਀                㰀琀搀㸀㰀⼀琀搀㸀 ਀                    㰀䠀㌀㸀䌀爀礀漀瀀爀攀猀攀爀瘀攀搀 倀䈀䴀䌀㰀⼀䠀㌀㸀

CTL also offers cryopreserved PBMCs that have pre-defined reactivity for the CEF ਀                        瀀攀瀀琀椀搀攀 瀀漀漀氀猀 愀渀搀 昀漀爀 琀栀攀 椀渀搀椀瘀椀搀甀愀氀 瀀攀瀀琀椀搀攀猀 漀昀 琀栀攀 瀀漀漀氀 愀猀 眀攀氀氀 愀猀 䠀䰀䄀 挀氀愀猀猀  I and II high resolution haplotype characterization. These cryopreserved PBMCs ਀                        愀爀攀 甀猀攀昀甀氀 昀漀爀 愀猀猀愀礀 搀攀瘀攀氀漀瀀洀攀渀琀Ⰰ 猀琀愀渀搀愀爀搀椀稀愀琀椀漀渀Ⰰ 愀渀搀 愀猀 戀愀猀椀挀 氀愀戀漀爀愀琀漀爀礀  research reagents.

਀                    㰀椀洀最 琀椀琀氀攀㴀∀吀栀攀 䔀䰀䤀匀倀伀吀 攀砀瀀攀爀琀猀∀ 猀爀挀㴀∀⸀⸀⼀椀洀愀最攀猀⼀挀琀氀ⴀ氀漀最漀ⴀ眀椀琀栀ⴀ愀搀搀爀攀猀猀⸀最椀昀∀㸀 ਀                㰀琀搀 眀椀搀琀栀㴀∀㐀　∀㸀㰀⼀琀搀㸀 ਀            㰀琀爀㸀 ਀                㰀琀搀 瘀䄀氀椀最渀㴀∀琀漀瀀∀ 眀椀搀琀栀㴀∀㤀㘀　∀ 挀漀氀匀瀀愀渀㴀∀㜀∀ 栀攀椀最栀琀㴀∀㄀　∀㸀㰀䤀䴀䜀 猀爀挀㴀∀⸀⸀⼀椀洀愀最攀猀⼀昀氀礀攀爀ⴀ戀漀琀琀漀洀ⴀ氀椀渀攀⸀最椀昀∀㸀㰀⼀琀搀㸀 ਀            㰀⼀琀爀㸀 ਀                㰀琀搀 挀漀氀匀瀀愀渀㴀∀㜀∀㸀㰀一伀匀䌀刀䤀倀吀㸀㰀䈀刀㸀 Back to the CTL home page and ਀                            洀漀爀攀 䔀䰀䤀匀倀伀吀 椀渀昀漀㰀⼀䄀㸀 ਀            㰀⼀琀爀㸀 ਀    㰀⼀戀漀搀礀㸀 ਀਀