Image Quality Can all ImmunoSpot® analyzers be used for double-color analysis?
Yes. The hardware and software of the ImmunoSpot® analyzers and the ImmunoScan™ readers all support single- and double-color analysis. The optics and cameras of the analyzers, however, are specifically enhanced for highest color fidelity and resolution.
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Other FAQ sections:
Assay Performance Scanning/ScAnalysis ELISPOT Analyzers ELISPOT Plates GLP compliance What is the image resolution of the ImmunoSpot® analyzers?
Our Analyzer allows 1600x1200 and 1200x1024 pixels per well, respectively.How is even illumination achieved in ImmunoSpot® analyzers?
ImmunoSpot® analyzers use a proprietary light configuration to evenly illuminate the entire well bottom surface. Best results will be achieved with non-reflecting (opaque) color-neutral (white) plates. In addition, an auto-light adjustment option which permit fine-tuning of the illumination for each well when the background varies strongly.Which types of assays might make high resolution necessary?
With spots in all size categories, high resolution images provide more information on the spot morphology, thereby increasing the accuracy of the advanced spot morphology recognition features offered by ImmunoSpot® software (SmartSpot™). The spot size reflects the per cell productivity and secretion kinetics. Also, the strength of T cell stimulation defines the spot size; low avidity T cell recognition results in low productivity and small, faint spots. Granzyme B, TNF and IL-10 assays frequently produce small spots. In all these cases, using high-resolution image analysis can provide a definite advantage.How large are the data files created in the scanning process?
For maximum accuracy, ImmunoSpot® analyzers can store its
data in loss-free Tagged Inline File Format (TIFF) files. At 512x512
resolution, the image of each well is a 660 Kb file: a single CD can
store the files for ten plates, and a DVD can store 70 plates. At
1024x1024 resolution, CDs and DVDs can store four and 30 plate images,
respectively.
In addition, the ImmunoSpot® analyzers also allow JPEG
images to be stored instead. The JPEG compression algorithm inherently
introduces some image distortion and loss of information; however, they
also require approximately 20 times less storage space.